Description: The Exonuclease-It™ plasmid purification system is designed to selectively digest linear chromosomal DNA contamination in a plasmid preparation, without significantly degrading any supercoiled plasmid (or any covalently closed circular plasmid). Exonuclease-It™ will also degrade open circular plasmid and circular single stranded DNA. The Exonuclease-It™ enzyme reagent contains a proprietary mixture of exonucleases to accomplish this task. In a typical 6 hour incubation, Exonuclease-It™ will reduce the amount of chromosomal DNA contamination in a plasmid preparation by 98 percent. The by-products of the exonuclease digestion are deoxynucleoside monophosphates. The Exonuclease-It™ plasmid purification system is ideally used as an added step in any plasmid purification method, in order to thoroughly remove chromosomal DNA. This kit contains sufficient reagents for purifying 150 mg of plasmid. Exonuclease-It™ is not for use in purifying plasmid for human therapy.

bayoubiolabs Biochemicals Catalog The lowest cost per loading DNA ladders in the world See the ladder summary chart below. DNA Ladders for conventional electrophoresis 20 bp DNA ladder 50 bp DNA ladder 100 bp DNA ladder 200 bp DNA ladder 500 bp DNA ladder 1 kilobase DNA ladder Supercoiled Plasmid ladder Vectors pUC and M13 DNA Ready to Ligate Vectors Ready to Ligate pUC18 and M13mp18 Molecular Biology Kits Supercoil-It™ Plasmid Purification Kit Exonuclease-It™ Plasmid Purification Kit Repair-It™ DNA Repair Kit Recombinant Enzymes Recombinant Enzymes